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Monday, May 18, 2020 | History

2 edition of Somatic hybrids and cybrids within Brassicaceae found in the catalog.

Somatic hybrids and cybrids within Brassicaceae

Eva Sundberg

Somatic hybrids and cybrids within Brassicaceae

studies focused on refining production methods and identifying factors influencing the genetic composition of somatic hybrids

by Eva Sundberg

  • 312 Want to read
  • 15 Currently reading

Published by Dept. of Plant Breeding, Swedish University of Agricultural Sciences in Uppsala, Sweden .
Written in English

    Subjects:
  • Cruciferae.,
  • Brassica.,
  • Brassicaceae -- Hybridization.

  • Edition Notes

    StatementEva Sundberg.
    The Physical Object
    Pagination124 p. in various pagings :
    Number of Pages124
    ID Numbers
    Open LibraryOL18004772M
    ISBN 109157645019

    Cytogenetic description of somatic hybrids According to Sun et al. (), genomic changes induced by genomic shock usually occur soon after the formation of hybrid cells. Either an increase or a reduction in DNA content or chromosome number has been reported for many regenerated somatic hybrids, including Solanum brevidens (+) S. Thirty-five chapters on various aspects of fusion of plant protoplasts and somatic hybridization deal with the regeneration of interspecific and intergeneric somatic hybrids and cybrids in various plants: cereals, grasses, legumes, potato, tomato, eggplant, lettuce, Brassica.

    Such hybrids have been obtained in tobacco, potato, Petunia and several other intergeneric and intertribal hybrids. (iii) Cybrids: Those somatic hybrids that involve normal protoplast of one species and nucleusless protoplast (cytoplast) of another species are known as cybrids. Such hybrids are useful in transfer of CMS from one species to other. Thirty-five chapters on various aspects of fusion of plant protoplasts and somatic hybridization deal with the regeneration of interspecific and intergeneric somatic hybrids and cybrids in various plants: cereals, grasses, legumes, potato, tomato, eggplant, lettuce, Brassica, Datura, Hyoscyamus, Nicotiana, Catharanthus, Rauwolfia, Citrus, Poncirus, Prunus, Pyrus, Populus, algae, bryophytes, and ferns.

    Somatic hybridization is the in vitro fusion of isolated protoplasts to form a hybrid cell and its subsequent development to form a hybrid plant []. This process includes three main steps. This process includes three main steps. of two different genomes followed by the selection of desired somatic hybrid cells and regeneration of hybrid plants (Evans DA and Bravo JE, ). It is an efficient way to produce hybrids by fusion of two different protoplast from different plants or species or varieties, and hybrids which produced by this way called somatic : Aliyi Robsa Shuro.


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Somatic hybrids and cybrids within Brassicaceae by Eva Sundberg Download PDF EPUB FB2

Recently, hybrids have been produced parasexually by protoplast fusion, between Brassica juncea (a major oilseed crop of the tropical world) and the sexually incompatible species Diplotaxis muralis and Erica sativa. The potential of somatic hybridisation in perennial tree breeding is best illustrated byFile Size: KB.

Somatic hybrids and cybrids within Brassicaceae: studies focused on refining production methods and identifying factors influencing the genetic composition of somatic hybrids.

[Eva Sundberg] Your Web browser is not enabled for JavaScript. This chapter describes a method of cytoplasm transfer within the brassicaceae family through Ca-PEG-mediated protoplast fusion. The method includes a protocol of nonmutagenic albinism induction based on spectinomycin-induced plastid ribosome deficiency (PRD).Cited by: 5.

Production of cybrids in Brassicaceae species. The somatic hybrids were selected using the properties of spectinomycin-induced plastid defects in “albino” O. violaceus plants (chloroplast.

ticularly within the Solonaceae, Umbelliferae, Rutaceae, Leguminoseae, and Brassicaceae. Somatic hybrids are normally produced by fusing protoplasts in specific chemical environments, or by electro- fusion, and then regenerating plants via an interme- diate callus step.

If protoplasts are differentially. Somatic hybridization and cybridization have great potential in plant improvement. In this chapter, types of somatic hybrids and cybrids, somatic fusion methods, selection schemes and.

Selection of Somatic Hybrids and Cybrids It is a very difficult to identify the hybrid cells microsopically, therefore, some type of selection technique are required at the level of culture to recover hybrid cell and its callus tissue following fusion.

Some important selection procedures are discussed below: 1. Auxin: The selection of hybrids of Nicotiana [ ]. Somatic hybrid: protoplasts from the cells of two species are taken and fused together.

Cybrid: a complete cell of a species is fused with the anucleated cytoplasm. Somatic hybrids don't have species barrier, where as cybrids are bound to species barrier.

Somatic Hybrids And Cybrids Assignment Help Somatic hybridization produces symmetric, asymmetric and cybrids. Symmetric hybrids contains the somatic chromosome complements of both the fusion parents while asymmetric hybrids have full somatic complements of one fusion parent and variable number of chromosome from the other fusion parent.

Somatic hybridization produces symmetric or asymmetric hybrids, or cybrids. Symmetric hybrids contain the somatic chromosome complement of both the fusion parents.

But asymmetric hybrids have the full somatic complement of one fusion parent and a variable number of chromosomes from the other fusion parent. Using somatic hybridization, Brassica napus was combined with 3 wild species: Eruca sativa [E. vesicara], a species from the same tribe as B.

napus, Thlaspi perfoliatum, from the tribe Lepidiae, and Barbarea vulgaris, from the tribe Arabidae, to evaluate different markers and methods for hybrid identification and characterization, and to trace the inheritance of alien DNA from donor species to Cited by: 2.

If the enzyme is dimeric (having two subunits), somatic hybrids usually contain an isoenzyme with an intermediate mobility properties. The isoenzymes are often variable within the same plant. Therefore, it is necessary to use the same enzyme from each plant (parents and somatic hybrids), from a specific tissue with the same age.

ADVERTISEMENTS: The following points highlight the six main screening strategies that are to be enforced for the selection of ideal somatic hybrids. The strategies are: 1. Microscopic Visual Selection 2.

Auxin Autonomy 3. Chlorophyll Complementation 4. Biochemical Selection 5. Verification by Molecular Screening 6. Chromosomal Analysis. Search within book. Front Matter. Pages I-XXV.

PDF. Protoplast Fusion, Somatic Hybrids, Asymmetric Hybrids, Cybrids — Transfer of Chloroplast Traits. Front Matter. Pages PDF. Somatic Hybridization — A Rich Source of Genetic Variability. Somatic Hybridization in Brassicaceae. Front Matter. Thus, rearranged mtDNA having species-specific fragments from both parents or novel fragments commonly occurring in somatic hybrids between species within Brassicaceae was difficult to detect in a cytoplasm combining B.

oleracea and B. napus by: These are the hybrids which are formed as a result of the somatic a result of somatic hybridization, the nuclear and the cytoplasmic fusion occur and it is known as the product is known as the somatic certain cases, only the cytoplasmic fusion occurs which may be followed by the loss of any one of the nucleus.

Thus the cell becomes uninucleated and such a fusion. Thirty-five chapters on various aspects of fusion of plant protoplasts and somatic hybridization deal with the regeneration of interspecific and intergeneric somatic hybrids and cybrids in a wide range of plants, including cereals, grasses, legumes, potato, tomato, eggplant, lettuce, Brassica, Datura, Hyoscyamus, Nicotiana, Catharanthus, Rauwolfia, Citrus, Poncirus, Prunus, Pyrus, Populus.

Somatic Hybridization and Applications in Plant Breeding Alexander A. Johnson Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA ‐, USACited by: 9.

• Advantages of cybrids • Need for cybrids • Protoplast isolation • Techniques involved • Cybrids in plant breeding 3. CYBRID • Cybrid – cytoplasmic hybrid. Cybrids are cells or plants containing nucleus of one species but cytoplasm from both the parental species.

• Cybridization – production of somatic cybrid. SOMATIC HYBRIDIZATION Development of hybrid plants through the fusion of somatic protoplasts of two different plant species/varieties is called somatic hybridization. This is a non conventional genetic procedure involving fusion between isolated protoplast under in vitro condition and subsequent development of their product (heterokaryon) to a.

Somatic Hybridization in Crop Improvement I. Authors: Bajaj, Y. P. S. Free Preview. Buy this book eBook ,13 Usually dispatched within 3 to 5 business days. Analysis of Somatic Hybrids and Cybrids Obtained by Fusion of Brassica rapa and B. oleracea. Somatic hybridization is generally used to induce disease resistance and improve certain qualities in the plants.

Pomato is one such example which is a hybrid of potato and tomato.Thirty-five chapters on various aspects of fusion of plant protoplasts and somatic hybridization deal with the regeneration of interspecific and intergeneric somatic hybrids and cybrids in various plants: cereals, grasses, legumes, potato, tomato, eggplant, lettuce, Brassica, Datura, Hyoscyamus, Nicotiana, Catharanthus, Rauwolfia, Citrus, Poncirus, Prunus, Pyrus, Populus, algae, bryophytes, and by: